Ipomoea pes-caprae is a medicinal plant used in many countries for the treatment of several ailments, including inflammatory and algesic processes. Invitro propagation of medicinal plants with enriched bioactive principle and cell culture methodologies selective for commercial production of medicinally important compounds. The increased use of plant cell culture systems, recent years, perhaps due to an improved understanding of the secondary metabolite pathway in economically important plant. Advances in plant cell cultures cost effective, commercial production of even rare or exotic plants, their cells and the chemicals that they will produce. This work indicates that the comparative study of antimicrobial activity of callus extract and adult plant extracts from Ipomoea pes-caprae. The plant was inoculated on Murashige and Skoog (MS) media containing different plant growth regulators (BA, 2,4-D, NAA+Kinetin, NAA+2,4-D+Kinetin) for the establishment of callus. Among this the combination of NAA+Kinetin (1.00+3.00mg/l) was found to be best for callus induction. Then this callus was sub cultured in a suspension medium containing different precursors (glutamine, KNO₃, FeSO_4,CuSO₄) for the population of secondary metabolite. The biomass produced in media supplemented with potassium nitrate 0.1 mg/l showed good antimicrobial activity against the test organisms. The result of this study supports the use of these plants for human and animal disease therapy and reinforces the importance of the ethno botanical approach as potential sources of bioactive substances.