In previous days people had erroneous belief that vitamin B₁₂ deficiency is improbable except in strict vegetarians or patients with pernicious anaemia. The concept was, therefore, that vitamin B₁₂ deficiency takes around 20 years for its stores to become depleted. The prevalence studies related to vitamin B₁₂ deficiency were thus underestimated in past. The prevalence studies, in last few decades, had reported that vitamin B₁₂ deficiency and depletion are common in wealthier countries, particularly among elderly. The clinical status of vitamin B₁₂ insufficiency is frequent in pathetic populations all around the world. However status of vitamin B₁₂ in people at eastern Nepal was unknown. Thus we carried out investigation in this area and observed that vitamin B₁₂ levels were below the reference range in approximately 21 % eastern Nepalese people. Among affected individuals, about 61.8 % females had undergone insufficiency states and 35 % young women in age-group 21- to 30-years-old were more prone to vitamin B₁₂ inadequacy. Hence we conclude that women suffering from vitamin B₁₂ deficiency in eastern Nepal are young  and is a concerned issue to health problem, the underlying cause of which has to be resolved.