Background: Human immunodeficiency virus (HIV) infection in children continues to occur globally. Early and periodical diagnosis is critical particularly in Sub-Saharan Countries. Forty three percent of Kenyan infants born to HIV infected mothers die before celebrating their second birth days. Objective:  To assess the extent and factors influencing utilization of HIV diagnosis among exposed infants attending Sigowet Sub- County Hospital, Kericho County. Design: A cross-sectional study  Setting: Sigowet Sub-County Hospital, Kericho County, Kenya. Subjects/Participants: One hundred and ninety nine HIV-positive mother-baby pairs attended to at the Hospital between January and March 2019. Results: Majority 198(99.50%) of the recruited participants were mothers of which 93(46.73%) aged between 18-26 years whereby nearly all infants were exposed to HIV infection .Study showed that 76(38.19) mothers had low education level while 107(53.77%) mothers had no formal employment. Study showed that 76(38.29%) mothers had secondary level of education while 107(53.77%) reported high rate of unemployment whereas a greater proportion 194(97.49%) mothers were exclusively breastfeeding for 6 months and 104(52.26%) mothers had never heard of HIV testing of infants. Study also showed that 107(53.77%) mothers only knew they were HIV positive during pregnancy while 44(22.11%) mothers did not disclose their HIV status to their husbands. However, 199(100.0%) mothers enrolled to care whereas 192(96.48%) mothers were not attached to any social group of People Living with HIV/AIDS (PLWHA). Correlation test of the individuals indicated that there was a positive correlation relationship of next of kin to HEI and age of mother, Level of education , marital status and number of children mother’s had( P=0.02, P=0.04,P=0.02 and P=0.01). There was a significant correlation between age of the mother and number of children (P=0.58 and P=0.92). There was a positive correlation between Mode of infant feeding to Level of education of the mother while a positive correlation between occupation of the mother and number of children (P=0.04, P=0.02 and P=0.18). However, there was a correlation in time of the mother’s awareness to her HIV status to the time of HIV status disclosure whereas a correlation between disclosure and attachment to social group of People Living with HIV/AIDS (P=0.03, P=0.01 and P=0.02).