Journal of Medical and Clinical Sciences

an Open Access Publication ISSN: 2455-3549 |

Solid Phase Extraction Using Polymer-Based C18 Cartridge Modified with Two Phenyl Azo Compounds for Spectrophotometric Determination of Mercury in Water and Environmental Samples

  • Alaa S. Amina


A new highly selective, sensitive, and rapid procedure to determine ng ml–1 level of Hg(II) depended on the speed reaction of Hg2+ with 5-amino-2-(phenyldiazenyl)phenol (I) and/or 5-amino-2-(p-tolyldiazenyl)phenol (II) and the solid phase extraction (SPE) of the colored complex with a reversed phase polymer-based C18 cartridge have been developed. The I and/or II reacted with Hg2+to form a violet complex of a molar ratio 2: 1 [I and/or II to Hg2+] in the presence of 3.0 M of nitric acid solution and Triton X-100 medium. This complex was enriched by SPE with a polymer-based C18 cartridge. The enrichment factor of 200 was achieved. The molar absorptivity of the complex are 8.81 x 108 and 9.57 x 108 L mol−1 cm−1 at 626 and 649 nm, using I and/or II, respectively Beer’s law is  obeyed in the range of 2.5 – 200 ng ml–1, whereas the optimum concentration ranges obtained from Ringbom plot was 10 – 175 ng ml–1. The detection and quantification limit are given as 0.75 and 2.37 ng ml−1 for I, whereas their values for II are 0.80 and 2.48, indicating that a nanogram fraction of Hg2+can be determined. The relative standard deviation was 1.45 and 1.34 % using I and II, respectively, obtained from a series of 10 standards each containing 100 ng ml−1 of Hg2+. This procedure was applied successfully to